Gene Analysis On Hypokalemic Periodic Paralysis

Object: Hypokalemic periodic paralysis has gene disorders in chromosomes.It can be a congenital condition or occasionally occurs as a result of a non-inherited genetic mutation that affects just the sperm or the egg cell. It was found that many patients were relatives in a family. So it was also called Familial hypokalemic periodic paralysis. Familial hypokalemic periodic paralysis is usually inherited as an autosomal dominant trait Many researches on it have found some mutated genes.There are two types : HypoPP1 and HypoPP2.In this experiment I analyzed a patient with hypokalemic periodic paralysis to find if he has any mutation of the mutations in code 528, 1239 in HypoPP1 and mutations in code 669, 672 in HypoPP2.Methods:To extract DNA from the patient's white blood cell in peri-blood by NaI method, do polymerase chain reaction.For different PCR product,using restriction fragment length polymorphism(RFLP) or single stranded conformational polymorphism(SSCP) technique. Then agrose gel electrophoresis or undenatured polyacrylamide gel electrophoresis to observe the results.Results:There is no different results in the patient from the controls in different four gene products of code 528, 1239 in HypoPPl and code 669, 672 in HypoPP2. Conclusion: The mutations have been studied were not found in the Hypokalemic periodic paralysis patient.