Angiotensin-â…¡ Increased The Calcium Deposition Of Vascular Calcification In Vitro

Objective To evaluate the effect of Ang â…¡ on calcium deposition and ALP activity in human vascular smooth muscle cells(HVSMCs) and investigate the mechanism of Ang â…¡ to inhibit calcification of HVSMCs.Methods The cell culture of human vascular smooth muscle was performed. p-glycerophospahte sodium salt was used to induce cultured HVSMCs to calcify and this serves as a vascular calcification model in vitro. Cells were divided into 4 groups: control, p-GP, Ang â…¡, β-GP+ Ang â…¡. 10^-7mol/L Ang â…¡ was added to culture media. Calcification was confirmed by vokossa staining. Calcium deposition of cellular layer, alkaline phosphatase(ALP) activity of intracellular was quantified during 10 days culture process. Cell cycle and apoptosis rate was analyzed.Results After 10d of culture , The calcium deposition in β-GP+ Ang â…¡ group was less than in p-GP group[(1.336±0.096) μmol/L versus (2.056±0.441)μmol/L, P<0.01]. The cellular ALP activity in β-GP+ Ang â…¡ group was less than in p-GP group[(691.27±128.06) IU/L versus (277.57±87.81) IU/L, P<0.01]. This process is time dependent. Flow cytometeric DNA analysis revealed that 10^-7mol/L Ang â…¡ cause significantly decreasing of apoptosis rate, descending of G₀/G₁ phase and increasing of S phase compared with calcified HVSMCs.Conclusions Ang â…¡ decrease calcium deposition of calcified HVSMCs. This may be through decreasing ALP activity, stimulaing proliferation and inhibiting apoptosis of HVSMCs.