Study On The Bone Repairing Materials And Porous Scaffold Based On Calcium Sulfate

Calcium Sulfate (CaS), as a restore material, shows biocompatibility, biodegradabilityand osteoconductivity. So the study about using CaS as repairing fillers for thetreatment of bone defection is reported now and then,but the fabrication of CaSporous scaffold, CaS/Gel, CaS/CS or CaS/Gel /CS composite biomaterials has notbeen reported, so we use calcium sulfate as the basic material to fabricate CaS porousscaffold and CaS/Gel, CaS/CS or CaS/Gel /CS composites as bone repairing materialsfor tissue engineering, and study the structure and characteristics of the materials byX-ray diffraction, IR and SEM.1. CaS/Gel, CaS / CS and CaS/Gel/CS composites were prepared. IR, X-ray and SEManalyses show that the crystal pattern of CaS is affected by adding Gel or CScomponents. The development of the CaS crystal is counteracted by adding Gel or CSand the crystal appearance has changed. The crystal appearance is affected bypolymer amount and sort, which can be used for adjusting the properties of thematerials.2. CaS porous scaffolds were prepared by using porogen technique and the surfacewas modified using polymers. The structure and characteristics were investigated. Inthe scaffold pores were inter-connected in the three-dimension and the pore size isover 200um. SEM shows that the appearance of CaS in the scaffold is different frompure CaS, which shows several appearances such as virgate, polygon etc. It was foundthat the density of the scaffold decreased and the porosity of the scaffold increasedwith increased porogen weight fraction. The surface of the pore modified withpolymer is better than the common porous scaffold.3. To evaluate their biocompatibility, MSCs were co-cultured with CaS composites invitro. CaS composites were implanted into the segmental radial bone defect in rabbit,in order to evaluate their degradability, bone regeneration and compatibility by meansof X-ray and histomorphological analyses.The result reveals that the composite biomaterials and porous scaffold are promisingin bone tissue.