| Objective: Heart failure is characterized by progressive myocardial remodeling an deteriorating cardiac function. And heart failure usually coexist with arrhythmia .at present, clinical medicaments against arrhythmia have some side-effect on negative contractility of cardiac niusle.more and more trial have been shown that Wenxin Keli can reduce arrhythmia, improve function of circulation. However, whether Wenxin Keli has the treatment effect on heart failure is unkown. We undertook this study in order to investigate Wenxin Keli has the treatment effect on isoproterenol( ISO) induced heart failure in rats .Methods: subcutaneous injection of ISO (Sigma , USA), 85 mg/ kg ,which wasadministered on two consecutive days (total of two injections) was employed to induce heart failure in rats . The control group received saline only.After 2 weeks.21 rats were measured up for heart failure model by echocardiogram measurement . Then.the rats were random assigned to six groups:(l) The rats in control group were only receive distilled water every day(n=10);(2) The rats in Valsartan and control group were receive Valsartan every day (n=10);(3)The rats in Wenxin Keli and control group were receive Wenxin Keli (9g/ kg)every day(n=10);(4) The rats in ISO group received two subcutaneous injections (85 mg/ kg) of ISO . which were separated by a 24 hour interval and began to receive distilled water 2weeks later every day(n=7);(5) The rats in Valsartan and ISO group received two subcutaneous injections (85 mg/ kg) of ISO , which were separated by a 24 hour interval and began to receive Valsartan 30mg/kg 2weeks later every day(n=7);(6) The rats in Wenxin Keli and ISO group also received two subcutaneous injections (85 mg/ kg) of ISO , which were separated by a 24 hour interval and began to receive Wenxin Keli (9g/ kg) 2weeks later every day(n=7). Rats were weighted Every week. Rats were evaluated by echocardiogram measurement after feeding medince 4weeks and 10 weeks, after feeding medince 10 weeks ,hemodynamic studies were performe in each group .After then, aconitine induced arrhythmia studies in rats were carried out.Results:Change of echocardiography:compared with control group, treatment with valsartan and Wenxin Keli in control groups , left ventricular internal diameter at diastolic phase(LVIDd),left ventricular internal diameter at systolic phase(LVIDs). LV percent fractional shortening(FS) and LV ejection fraction (EF) were not changed .LVIDd and LVIDs were increased , FS and EF were decreased in the ISO group .ISO group treatment with valsartan 4 weeks later , LVIDd and LVIDs were also increased. FS and EF were decreased compared with the control group .10 weeks later. LVIDd were increased , LVIDs * FS. EF were not deference compared with the control group . ISO group treatment with Wenxin Keli 4 and 10 weeks later . LVIDd and LVIDs were increased , FS and EF were decreased compared with the control group, compared with ISO group, ISO group treatment with valsartan 4 weeks later , LVIDs were decreased, FS and EF were increased .10 weeks later, LVIDdx LVIDs were decreased, FS, EF were increased . ISO group treatment with Wenxin Keli 4 weeks later , LVIDd, LVIDs. FS and EF were not deference compared with the ISO group . 10 weeks later, LVIDd, LVIDs, FS and EF were yet not deference compared with the ISO group.compared with valsartan and ISO group, ISO group treatment with Wenxin Keli and valsartan 4 weeks later . compared with Wenxin Keli and ISO group , LVIDs were decreased, FS, EF were increased obviously in valsartan and ISO group . 10 weeks later, LVIDd, LVIDs, EF, FSwere also improved obviously than ISO group treatment with Wenxin Keli.2. Change of hemodynamics: compared with control group, treatment with valsartan and Wenxin Keli in control groups , left ventricular end diastolic pressure(LVEDP). left ventricular systolic pressure(LVSP). ±dp/ dtmax were not changed obviously after 10 weeks . LVEDP was increased , LVSP and ±dp/ dtmax were decreased after 10 weeks in ISO groups .ISO group treatment with Valsartan . LVEDP was decreased . LVSP and ±dp/ dtmax were increased after 10 weeks . treatment with Wenxin Keli in ISO group, LVEDP was decreased. ±dp/ dtmax were increased after 10 weeks . compared with Valsartan and ISO group, WenXin KeLi and ISO group LVEDP was increased . LVSP was decreased .but ±dp/ dtmax and HR were not obviously deference.3. Aconitine induced arrhythmia : treatment with Wenxin Keli, Wenxin Keli and control group were less serious than those in control group, aconitine induced arrhythmia in rats in ISO group were more serious than those in control group, aconitine induced arrhythmia in rats in Wenxin Keli and ISO group is also more serious than those in control group but less serious than those in ISO group.However.valsartan could't show its protection on aconitine induced arrhythmia both in control group and ISO group.Conclusion:(1) Wenxin Keli could a certan extent improve the ISO induced cardiac dysfunction;(2) Wenxin Keli could protect aconitine-induced arrhythmia in rats . |
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