Two-dimensional Electrophoresis Establishment And Analysis On SHR Rat Renal Tissue And Its Effects By Losartan

Hypertension is regarded as a disease of high incidence,high mutilation rate and high mortality .Now there are more than 100,000,000 sufferers in China and more than 50,000,000 sufferers in American.The goal of hypertensive therapy not only lies in depressurization but in protection of target organs,such as kidney.It is indicated that renin-angiotensin system (RAS) play important roles in renal damage and regulation of blood pressure.ACE is a key enzyme in RAS, which can promote angiotensin II and degrade bradykinin. After inhibiting to angiotensin II receptor, Angiotensin II receptor antagonist (ARB) acts as lowering blood pressure,protecting heart and kidney .At present, information about pathogenesy of high blood pressure influencing renal and the roles of Angiotensin II receptor antagonist is limited.Proteomics is a new subject from genomics and mainly studies proteins of cells and its regulation, including characterization of proteins, post-translational modifications and the interation of proteins.As main separation technique, Two dimensional gel electrophoresis can separate tens of thousands proteins.It makes possible to observate the influence of Losartan to renal damage from high blood pressure.Male SHR rats were used in this study.All rats were divided into two groups, spontaneous hypertensive rats (SHR, n=4), as hypertensive control, SHR +Losartan (SHR-L, n=4) as SHR-L group.Two group rats were breed for 8 weeks in the same circumstances. Losartan tablets dissolving into water were fed to SHR-L group with 20mg/ kg /day.The same volume water was fed to SHR group per day.Rats were put to death after 8 weeks. Two dimensional gel electrophoresis was performed after kidney total protein extraction and quantification. We obtained the atlases of the samples of the two groups by the technique of silver stain.After data acquisition and image analysis by Imagemaster 2D v5.0 computational software,we got a differential expression altas for each group and then matched them.The results showed: the number of average protein spots of two groups were 570±48 vs 686± 30 respectively. Average matching rate: 74.05% vs 65.9%.After qualitation and quantitation analysis for the atlases, we got 13 differentially expressed proteins of two groups. There were 5 protein spots detected only in SHR group,while 4 protein spots were detected up-regulately and 4 spots down-regulately in SHR-L group. It was tested that a series of kidney proteins had changed after Losartan treatment. We conclude that kidney protection from Losartan involve multiple procedures or multiple factors.Conclusion: Great changes of kindey proteins occurred after Losartan were used to treat hypertensive rats. Then identification helped us thoroughly understand kindey protection and its mechanism of angiotensin II receptor antagonist.lt can provide clues for searching pre-markers to reflect Losartan's effective protection to kidney affected by hypertension.