Studies Of The TGF-β1-Responsive Drug-Resistance In Ductal Adenocarcinoma Of Pancreas

Researches have demonstrated that mdrl was the major reason of Multiple-drug resistance in pancreatic carcinoma. PKC α acts as an important modulator of mdrl. Our previously study has found TGF β 1 can up-regulating PKC α protein. In order to demonstrate the MDR mechanism of pancreatic carcinoma,we use tissue arrayer and immunohistochemistry to observe the relationships among the expressions of TGF β 1,PKC α and P-gp proteins. At the same time we detected the expression of PKC α and P-gp in pancreatic carcinoma cells(BxPC3) with Western-blot after stimulated with TGF- β 1. MTT assay was used to observe the sensitivity of BxPC3 cell to anti-cancer drug cisplatin to detect the influence of TGF- β 1 .Conclusions: Overexpression of TGF- β 1 in pancreatic carcinoma may contribute to the membrane tranfer of PKC α .TGF- β 1 can enhance the expressions of PKC α and P-gp proteins in pancreatic cancer cell line BxPC3 and may aggratate the drug-resistance of pancreatic cancer cells.