Neuroprotective Effect Of Minocycline On Rat Parkinson Disease Model Induced With LPS

Objective: To establish the rat Parkinson disease (PD) model induced with lipopolysaccharide (LPS) and study LPS-induced injury of dopaminergic neurons.Methods: Lipopolysaccharide was stereostatically injected into uni--lateral substantia nigra of rats. The circling behavior was observed by intraperitoneal-injection of apomorphine during the intervals of 1 , 2 and 4 weeks after injection. Tyrosine hydroxylase (TH) immunohisto--chemistry was used to detect midbrain dopaminergic neurons. RT-PCR was used to detect expression of TH mRNA.Results: The circling behavior ipsilateral to LPS injected-side was induced in some rats 2 and 4 weeks following injection , but not in 1 weeks . The marked loss of TH-positive cells in the injured substantia nigra was identified , especially 4 weeks after LPS treatment. The de--creased expression of TH mRNA in the injured substantia nigra was iden-tified , especially 4 weeks after LPS treatment. Conclusions: LPS intranigral injection could injure the dopaminergic neurons in the substantia nigra. The rat PD model induced with LPS was established successfully. It may be the best rodent model of PD appling...