Practical Research On Increasing Blood Viscosity Model's Animal And Its Serum Causes The Toxicity Of The Brain Cells

Objective1.To investigate the correlation between blood viscosity and oxygen content of arterial blood in increasing blood viscosity rats model. 2.To observe cell apoptosis and the changes of cytosolic free calcium concentration by interfering cerebral cells with serum of increasing blood viscosity rats model. Discussing the possible causing-damage factors in the model serum.Methods:1. Animal model of increasing blood viscosity was established by intravenous injection of high molecular dextran to rats. The visible components and hemorheology were detected using cone-plate viscometer and automatic programming coagulation analyzer.2.Detecting the blood oxygen saturation and blood oxygen pressure changes in increasing blood viscosity rats model's internal jugular vein and carotid artery with i-STAT clinical blood analysis system.3.Interfereing rats'brain cells with the serum from the increasing blood viscosity rats model at different time points. Using Ca²⁺ specific fluorescent probe Flou-3/AM as fluorescent indicator, to detect the cytosolic Ca²⁺ changes with the laser confocal scanning microscope.4.Applying acridine orange and propidium iodide as stains, to observe brain cells apoptosis and death conditions with different periods cultured by the serum of increasing blood viscosity model rats with five-day stablishing models under the laser focusing microscope.Results1.Model-forming time is positive correlation with blood viscosity and plasma viscosity. And it is negative correlation with the reduction of blood visible components.2.The model-forming rats blood viscosity and plasma viscosity is negative correlation with oxygen content of arterial blood(CaO₂), venous oxygen saturation(SjvO₂), partial venous oxygen pressure(PvO₂) and oxygen content of venous blood(CjvO₂). And there is no correlation with arterial oxygen saturation(SaO₂) and arterial partial pressure of oxygen(PaO₂).3.Compared with control group, there was a fluctuation of Ca²⁺ concentration in brain cells interfered by the serum of model rat which formed for 20 minutes, and there was a persistent calcium overload in cerebral cells interfered by serum of model rat which formed for 5 days.4.Apoptosis and death of brain cells happened in 30 minutes which interfered by serum of rat model which formed for 5 days. As the interfering time goes by, apoptosis and death of cerebral cells seemed to be much more serious.Conclusion1.The establishing method of increasing blood viscosity model animal by DT is convenient, simple and repeated, and the achievement ratio is high.It is a optimum optimum method to chromic deficiency of blood and oxygen.2.Arterial oxygen(CaO₂) content can be used as a evaluating standard of degree of the hypoxia to DT-making increasing blood viscosity rat models.3.The serum of increasing blood viscosity animal model which formed for 5 days has factors to damage to rat cerebral cells, which factors will do some further research.