Study Of MMP-2, E-cadherin Expression In Laryngeal Carcinoma And Their Relation With Neck Lymph Node Metastasis

Owing to plentiful lymphatic tissue,neck metastasis iscommon in supraglottic carcinoma.So when the primarylesion is excised,the treatment of the neck is usuallynecessary.As a result,the accurate prediction of the necklymph node before procedure is very important.To observe MMP-2 and E-cadherin expression and theirrelationship with lymphatic metastasis in supraglotticcarcinoma,48 cases were detected using immunohistochemistry.1. MMP-2 expression and its relationship with lymphaticmetastasis in patients with supraglottic carcinoma10 cases of normal mucosa and 48 cases of supraglotticcancer were detected, no expression was observed in normalmucosa . MMP-2 expression rate was 66.7% in tumor tissue,it was immunolocalized on the cytoplasm.Strong staining ofMMP-2 was observed in the front of the tumor,indicatingtheir strong invasive ability through the degradation oftype â…£ collagen.Compared with group without lymph nodemeatstasis the MMP-2 expression was much higher in groupwith lymph node metastasis.MMP-2 play a key role in theinvasion and metastasis of supraglottic carcinoma oflarynx.2.E-cadherin expression and its relationship with lymphnode metastasis in supraglottic carcinoma.10 cases of normal mucosa and 48 cases of supraglotticcancer were detected,E-cadherin was strongly stained inall the 10 normal laryngeal mucosa. positive expressionrate of E-cadherin was 52.1% in tumor tissue.It wasimmunolocalized on the membrane.In tumor tissue withstrong staining of E-cadherin,tumor cells infiltrate thenormal tissue in the form of mass,while cluster or diffusetumor cells in tumor tissue with poor or no expressin ofE-cadherin.We speculate that downregulation of E-cadherinresults with the downregulation of the adhesive abilitybetween tumor cells,thus these tumor cells can escape theprimary lesion and metasize to the lymph vesseleasily.Compared with group with lymph node metastasisE-cadherin expression rate was much higher in group withoutlymph node metastasis.It can be speculated that thedownregulation or absence of E-cadherin was significantlyrelated with the lymph node metastasis of supragoltticcarcinoma.3.The correlation between the expression of MMP-2 andE-cadherin in supraglottic carcinomaMMP-2 and E-cadherin was negatively correlated insupraglottic carcinoma.4.MMP-2,E-cadherin expression and the prediction of lymphnode metastasis of supraglottic carcinomaIn our test, we predicted neck metastasis usingMMP-2(+),E-cadherin(-),and MMP-2(+) with E-cadherin(-) asindex repectively.The result showed that MMP-2(+) had thehighest sensitivity,but lowest specificity and positivepredictive value.On the contrary, MMP-2(+) withE-cadherin(-) had the lowest sensitivity but highestspecificity and positive predictive value. E-cadherin(-)was intermediate.Acted as two important proteins in theinvasion and metastasis of the tumor,MMP-2 and E-cadherinare reliable markers for the prediction of lymph nodemetastasis of supraglottic carcinoma.Combined detectionof MMP-2 with that of E-cadherin,we can boost the accuracyof the prediction of neck lymph node metastasis insupraglottic carcinoma.