Study On Antibiotic Resistance Of Staphylococcus Aureus And Genotyping Of Methicillin-Resisitant Staphylococcus Aureus

Objective: To investigate antibiotic resistance and distribution ofPanton-Valentine leucocidin (PVL) of Staphylococcus aureus isolatesrecovered in Changchun hospitials between June in 2005 and January in2006;methicillin-resistant Staphylococcus aureus (MRSA) wereidentified and characterized using SCCmec typing. Methods: Antibioticresistance was studied by agar dilution method and antibiotic paperdiffusion method (K-B). Pulsed-field gel electrophoresis (PFGE) wasused to type the chromosome DNA of MRSA strains. SCCmec typing(I-V) of all clinical isolates of MRSA was performed by novel multiplexPCR assay. Results: The resistant rate of Penicillin G, Clindamycin andTetracycline were 96.4%, 55.4%, 51.8% in SA. The sensitivity rate ofTrimethoprim/ Sulfamethoxazole, Chloramphenicol and Rifampin were97.6%,91.6% and 90.4% in SA. 100% MRSA strains were susceptibleto glycopeptides such as vancomycin and teicoplanin. The majority(58%) of the strains were resistant to erythromycin, Gentamicin,Tetracycline, Ciprofloxacin, Levofloxacin, Gatifloxacin, Moxifloxacin,Clindamycin, Rifampin. The PVL genes were not found in MRSA.majority of the isolates (75%) had SCCmec type III. Conclusions: Thisstudy has demonstrated that MRSA isolates recovered in Changchunhospitials between June 2005 and January 2006 in Jilin province wereHA-MRSA.