The Expression And Clinical Significance Of CD40 And ICAM-1 In Carcinomas Of Liver

Hepatocarcinoma is the most common type of malignant tumors,with uncertain prognosis, and urgently necessitates the development ofalternative therapeutic plans. Gene treatment is regarded as apromising measure.The experiment in this study examines theexpression of elements CD40 and ICAM-1 in live cancer tissues witha purpose of exploring the relationship between their expression andthe formation, development, and prognosis of hepatocarcinoma inorder to provide experimental evidence for biological and clinicalresearch.Forty pathologic specimens of hepatocarcinoma have beencollected from the resectional therapy of the surgery operation at theFirst and the Second Hospitals of Jilin University from April 1998 toDecember 2005. Of these specimens, 25 cases are with tissues near thecancer tumor (distance from cancer tissues ≥ 2 cm). Prior to theoperation, patients have not received anti-cancer treatment;followingthe operation, resectional therapy has proven these patients to havedeveloped cellular liver cancer. All specimens have been fixed withthe conventional 10% formaldehyde, dehydrated with an ethanolgradient, made transparent with dimethylbenzene, and embedded withparaffin.The preparation of 4-μm consecutive sections is completedwith routine HE and immunohistochemical staining which, in turn, isachieved using the hypersensitive S—P method. During the process,PBS replaces primary antibodies in the negative control and knownpositive sections are used as positive control. The acquired results arestatistically analyzed with SPSS software;the data between groups areexamined with Fisher's exact probability test.Tumor occurrence and metastasis involve many factors and arecomplex immunobiological processes. In recent years, many researchfindings indicate that CD40 is central to specific immune responses.The abnormal expression of CD40 in a series of epithelial tumortissues is highly relevant to the biological behaviors of tumor invasion,metastasis, diagnosis, and prognosis. However, CD40 aglycone andprovocation can interfere and result in growth inhibition and death oftumor cells, and plays an important role against infection, viruses, andatherosclerosis. Moreover, CD40 is significant to the immunization ofthe body fluid and of the cell.The multiple antigens, receptors and other molecules on thesurface of the tumor cells are not isolated, but act as a coordinatedentity. CD40 and its antibodies or recombined ligands play an essentialrole in increasing the level of ICAM-1 on the surface of thehepatocarcinoma, heightening the expression of FAS, andstrengthening the sensitivity to death when FAS interferes. Also,ICAM-1 is an adhesive element central to the cell's immune response,mainly acting on promoting the adhesion between heterogeneous cells,causing the interaction between different cells and resulting in themetastasis of the tumor.This experimental study indicates that positive expression ofCD40 and ICAM-1 in liver tissue is higher than that in the controlgroup, with a statistically significant difference (p<0.01). The resultsdemonstrate that the combination of CD40 and its aglycone canimprove the expression of ICAM-1 at the cell surface. The abnormalexpression of CD40 and ICAM-1 in liver cancer tissues is relevant toits occurrence, development, and metastasis. In addition, the studysuggests that CD40 and ICAM-1 expressed in liver cancer tissues hasan effect on the formation, development and metastasis ofhepatocarcinoma, and can be used as a criterion in determining thediagnosis of hepatocarcinoma, its development, metastasis, andprognosis. Due to the fact that the stimulation of element CD40 cannot only be used directly in liver tumor cells, but its signal can alsoaffect the formation and development of hepatocarcinoma at varioussteps of the cascade. Due to these results, interference with the signalof CD40 can be used as a new target and strategy in immunity againstand treatment of hepatocarcinoma tumors.