| Neuroblastoma is one of the most common malignancies in childhood, and is also one ofthe most difficult to treat. It is an embryonic tumor, which derived from neuronal crest.Neuroblastoma has the highest rate of spontaneous regression among human tumors, andreagents can also induce neuroblastoma differentiation. Neuroblastoma cell line (SH-SY5Y)is a good model for neuronal development, and the molecular mechanism of neuronaldifferentiation can be discovered, which can avail to clinicians. Retinoic acid (RA) is one ofthe most potent differentiation inducers. Treatment of neuroblastoma cell lines with all-transretinoic acid causes significant proliferation inhibition and sometimes neurite outgrowth. Nowit is known that most of the action of retinoic acid can alter gene expression. Microarraytechnology allows relatively rapid and simultaneous identification, comparison, and analysisof thousands of genes, which is important to understand molecular mechanism of tumor.To monitor gene expression profiles during the progress of neuroblastoma differentiationat a genome scale, we aquired 209 genes deriving from the embryonic brain and differentkinds of nervous tumors, which were subsequently used to construct a oligonucleotidemicroarray. This microarray was used to analyze gene expression in neuroblastomadifferentiation by RA 1-8 day. After analysed, the data showed that there were 13 up-regulatedgenes, but no down-regulated genes. Five genes-Mab21l2, protein4.1N, SCG10, PS2 andGAS6-were identified and verified by semi-quantitative RT-PCR. Microarray andsemi-quantitative RT-PCR analyses showed a high correlation between the two methods. Ourresults indicate that the oligonucleotide microarray is reliable and can be used for monitoringgene expression profiles in the progress of neuroblastoma differentiation. In addition, thedifferent express of genes during differentiation represent candidate genes for dissectingmolecular mechanism of neuroblastoma differentiation.
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