The Study Of Hyposensitization Of Allergen Nebulization In The Prevention And Treatment Of Bronchial Asthma

Obiective:To explore the role of hyposensitization of allergen nebulization in the prevention and treatment of bronchial asthma,to understand the function ofγδT cells in the pathogenesis of asthma,to understand the immune mode ofγδT cells.Methods:Twenty patients with Der.p and Der f allergy and chronic severe persistent asthma participated in a randomized study.All patients were treated with 50/250μg salmeterol xinafoate and fluticasone propionate(trade name: seretide) twice a day for six months.At the same time,group A were nebulized with 5ml normal saline while group B with specific allergen twice a week for six months.The patients'clinic synthesis indexes were evaluated after therapy and before.The percentage ofγδT cells in peripheral blood and induced sputum and the percentage of IL-4 positiveγδT cells and IFN-γpositiveγδT cells were detected by flow cytometry after therapy and before.Results:Asthma symptoms,lung functions were improved distinctly,no changes of skin pick test(SPT) intensity between two groups,the presses of ventolin uses were decreased markedly between two groups than before,but group B were excelled in symptoms and more less uses of ventolin than group A. The percentage ofγδT cells decreased and the percentage of IFN-γpositiveγδT cells increased clearly than before in group B in both peripheral blood and induced sputum.Conclusion:γδT cells,the important cells in the pathogenesis of bronchial asthma,may have Th1/Th2 immune mode.The predominant expression ofγδT...