| Objective: to study the anti-tumor effect and its mechanisms of Camellia chrysantha (Hu) Tuyama and ginkgo biloba leaves in the rat's liver preneoplastic nodules induced by DEN and in human hepatocellular carcinoma cells (BEL7404).Methods:In vivo study(Animal trial): The male rats, 6 weeks old, were used for establishing a short-term preneoplastic model: 121 male wistar rats administed Diethylmitrosamine(DEN)200mg/kg.bw by abdomen injection. Then 0.015% 2-AAF solution which was mixed into the normal food was administered by the rat for two weeks. Two-thirds partial hepatectomy was performed after seven days for taking 2-AAF. The rats were divided into five groups randomly: 20 rats for group A, the flowers of Camellia chrysantha (Hu) Tuyama (CCT)was administered; 20 rats for group B, the leaves of CCT was administered; 20 rats for group C, the concentrated liquids of CCT was administered; 20 rats for group D, Ginkgo Biloba leaves (GBL) was administered; 41 rats for group E as the control, the normal food were administered. The third day after stopping feeding 2-AAF, all the rats were executed. Five pieces of liver were taken from each rat. The preneoplastic nodules forγ-GT positive foci in liver sections were measured, and calculated the total areas of the positive foci per square centimeter (mm~2/cm~2), the average acreage per positive foci (mm~2/entries), and the amount of positive foci per square centimeter (entries/cm~2) respectively.
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