To Investigate The Relationship Of Hepatitis B Virus Genotypes,Mutations In The Hepatitis B Virus Genome With HBVDNA Rebound After Lamivudine Therapy.

AIM:To investigate the relationship of Hepatitis B virus genotypes,mutations in the hepatitis B virus(HBV) genome and mutated spot with HBVDNA rebound after with lamivudine therapy.METHODS : To inspect twenty-seven patients (therapy group, HBVDNA>104copie4/Ml) of HBVDNA rebound after respond to virus beyond 6 months with infecting hepatitis B received lamivudine ( 100mg/day) oral application for at least 12 months and nineteen patients (control group) with infecting hepatitis B without treated with the drug of virus, examined the Hepatitis B virus (HBV) genotypes of them by nested PCR with multiple pair primers. Examined mutations in the Basal Core Promoter(BCP), Precore(PC), Core Genes and Polymerase Genes(YMDD) of two group by PCR-gene sequence analysis .RESULTS: (1) 13 patients were detected YMDD mutation in 27 patients of HBVDNA rebound in therapy group(HBVDNA _negative group). YMDD mutation was not detected in control group and therapy group 4 case serums before therapy, P<0.01. The location of the YMDD mutation was rtM204I/V (domain C)±rtL180M(domain B) according to the new nomenclature. (2) The rate of BCP(A1762T + G1764A) mutations were 44.44%(12/27) in HBVDNA- negative group beyond control group 6.32%(5/19) ,but not difference ,P>0.05 . (3) 9 to 27 patients (33.33%) of HBVDNA- negative group were detected PC(G1896A) mutation, Compared with control group(5.26%), P<0.01. C1856T mutation was not difference in therapy and control group. (4)Core gene...