Observation Of Immune Responses After Intranasal Immunization With Toxoplasma Gondii RH Strain Excreted/secreted Antigens (ESA)

Objective The primary aim of this study was to investigate the mucosal and systemic immune responses after intranasal immunization with Toxoplasma gondii RH strain excreted/secreted antigens (ESA) taken from the infected mice peritoneal fluids and determine the optimal antigens of ESA .The next goal was to study the mucosal and systemic immune responses after intranasal immunization with Toxoplasma gondii RH strain excreted/secreted antigens (ESA) taken from culture medium containing tachyzoites in vitro and determine the optimal antigens of ESA and observe duration of immune responses after intranasal immunization with the optimal antigens of ESA.Methods This study included three parts. The first part was to study the immune effects after intranasal immunization with Toxoplasma gondii RH strain excreted/secreted antigens (ESA) taken from the infected mice peritoneal fluids and determine the optimal antigens of ESA. Toxoplasma gondii RH strain ESA were administrated by the intranasal route, to four groups of 40 BALB/c mice for 3 weeks. three group received with 24h,48h,72h and 20μg per mouse twice at an internal of 2 weeks, while mice intranasally administrated with PBS in the same means were control. The condition of mice about health and death was observed and the weight of mice was recorded every day. Mice were killed on the third week after the last immunization. Serum IgG and sIgA in feces were detected by ELISA. The spleens were weighed and the number of the Peyer's patches was counted. Lymphocytes in PP, spleen, IEL, MLN were isolated and counted.The second part was to study the effects of intranasal immunization with Toxoplasma gondii RH strain excreted/secreted antigens (ESA) taken from culture medium containing tachyzoites in vitro and determine the optimal antigens of ESA. Toxoplasma gondii RH strain ESA were administrated by the intranasal route, to six groups of 60 BALB/c mice for 5 weeks. five groups received with 5d, 7d, 9d, 11d, 14d ESA and 20μg per mouse twice at an internal of 2 weeks, while mice intranasally administrated with culture medium except tachyzoites in the same means were control. The condition of mice about health and death was observed and the weight of mice was recorded every day. All of the mice were killed on the third week. Serum IgG and sIgA in feces were detected by ELISA. The spleens were weighed and the number of the Peyer's patches (PP) was counted. Lymphocytes in PP, spleen, MLN and IELwere isolated and counted.The third part was to observe the mucosal and systemic immune responses after intranasal immunization with Toxoplasma gondii RH strain excreted/secreted antigens (ESA) taken from...