The Expression And Clinical Application Of PI3K, Akt And MTOR In Leukemia

Objectives: The phosphatidylinositol 3-kinases (PI3Ks) are a family of enzymes that produce lipid second messengers(such as PIP2 and PIP3) by phosphorylation of plasma membrane phosphoinositides at the 3-hydroxyl of the inositol ring. Akt could be recruited to the membrane by PIP3 that is one of the lipid second messengers. Akt, mTOR are activated in turn. The activated pathway control many cellular processes that are important for the formation and progression of cancer including various subtypes of leukemia. Several studies showed that inhibition of the PI3K/Akt/mTOR pathway might provide benefit for the prevention and treatment of leukemia. Here, we studied the expression of PI3K, Akt and mTOR in acute leukemia and chronic myelocytic leukemia. At the same time, we tried to find out a relationship between the prognostic significance of acute leukemia and the expression level of PI3K, Akt and mTOR.Methods: The expression of PI3K, Akt and mTOR mRNA were measured by semi-quantity reverse transcription polymerase chain reaction(RT-PCR). The samples were collected from 63 de novo acute leukemia(AL) patients, 7 replased AL patients, 14 chronic granulocytic leukemia(CML)...