Protective Effect Of Dipfluzine Against Lead-induced Injury On Cultured Primary Hippocampal Neurons

Lead could affect neural functions, mostly intellectuality development of infant as well as learning and memory of small-fry. Learning and memory is a complicated process, the cellular and molecular mechanism of which has not been clarified yet. But it is well accepted that hippocampus is the pivotal site relating to the ability of learning and memory.Dipfluzine (Dip), a new calcium antagonist, was first synthesized by Hebei Medical University. Its lipophilic quality enables it to permeate the blood-brain-barrier and achieve effective drug concentration in the cerebrospinal fluid. Dip could inhibit calcium influx and maintain intracellular calcium homeostasis of cardiac myocyte. It could improve cerebral microcirculation and supply of energy and nutrition in cells, amend the amnesia induced by sodium nitrite and sodium pentobarbital in mice and protect the ischemic brain in rat by reducing the disturbance of cortical function and the imbalance between excitatory and inhibitory amino acids. Thus it could be presume that Dip could be a protector to lead-induced injury of hippocampal neurons.The present study aims at observing the protective effects...