Effects Of VEGF₁₆₅ On The Vascularization Of Acellular Dermal Matrix

Objective: For improving the quality of acellular dermal Matrices (ADM), to study the biocompatibility of ADM prepared by SDS and NaOH maceration and the effects of transgene VEGF₁₆₅ on the vascularization of ADM.Method: The epidermis was removed after the mature fetus's skins being treated with a hyperosmotic salt solution. Subsequently, the dermis was macerated by the 4% aqueous solution of NaOH and the 0.5% aqueous solution of SDS for 10 hours respectively. During this period, ultrasonic wave shaking was intermittently used to remove the cellular components from the dermal matrix completely. ADMs were dehydrated and embed by paraffin conventionally and characterized them with HE staining to observe their inner general structure and cells components.ADMs of 300 μ m were cut by cryo-microtome and conservate them in PBS.Randomly 45 mice were devided into three groups-ADM vacnity group,ADM+rAAV/LacZ control group and ADM+rAAV/VEGF 165 experiment group.ADMs were embed between skins and skin-muscles on the backs of mice.2,4,6 weeks after operations mice skins were drawn the materials from back section.Subsequently,skins were fixated by Bouin solution,dehydrated,embed by pqrqffin and cut by...