The Research Of Antitumor Effect And Its Mechanism Of Fuzhengxiaoliutang

OBJECT:the experiment carried through elementary discuss in allusion to the inhibitory carcinom of Fuzhengxiaoliutang and correlative mechanism .METHODS:(1) In the inhibitory experiment of the solid carcinom:the model of micebearing carcinoma was established and calculate the inhibition ratios of the tumor weight.Repeat it three times.(2) In the experiment of prolonging the Kunming mice bearing H22:the model of mice bearing carcinoma was established by inoculating H22 cells into the abdominal cavity of the Kunming mic.calculate the live days and the rate of prolonging live.Repeat it three times.(3) In the experiment of the effect on IL-2 content in serum and spleen of the Kunming mice bearing sarcoma-180 ceils:Establish the model of micebearing carcinoma.adopt ELISA to mensurate IL-2 content in serum and spleen.(4) In the experiment of lymphocyte transform:Establish the model of micebearing carcinoma.Adopt morphologic check-up method to mensurate lymphocyte transform degree that PHA induced.RESULTS:(1) In the inhibitory experiment of the solid carcinom:By Stating the average weight of the each group solid carcinoma,it indicated that every therapy group can inhibite the solid carcinom in some sense.compared with control group,the group- group distinctions have statistics meaning.medium dose group and low dose group of Fuzhengxiaoliutang decoction has better effect.(2)In the experiment of prolonging the Kunming mice bearing H22:calculate the live days and the rate of prolonging live,it indicated that Fuzhengxiaoliutang can-prolong the lives of the Kunming mice bearing H22,compared with control group,the group-group distinctions have statisticsmeaning.low dose group of Fuzhengxiaoliutang decoction has better effect.(3) In the experiment of the effect on IL-2 content in serum and spleen of the Kunming mice bearing sarcoma-180 cells: Fuzhengxiaoliutang decoction can increase IL-2 content in spleen of the Kunming mice bearing sarcoma-180 cells,compared with control group,the group- group distinctions have statistics meaning.but it has no effect on IL-2 content in serum.However cyclophosphamide group has low IL-2 content in comparetion with control group.(4) In the experiment of lymphocyte transform: Fuzhengxiaoliutang decoction can accelerate lymphocyte transform degree that PHA induced.compared with control group,the group- group distinctions have statistics meaning. However cyclophosphamide group has low rate of lymphocyte transform contentin comparetion with control group.CONCLUSION^ 1) Fuzhengxiaoliutang can inhibite clearly sarcoma-180.(2)it can prolong the lives of the Kunming mice bearing H22 in the abdominal cavity.(3)it can increase IL-2 content in spleen of the Kunming mice bearing sarcoma-180 cells and can accelerate lymphocyte transform degree that PHA induced.