Investigation Of High Performance Liquid Chromatography With Chemiluminescence In Pharmaceutical Analysis

This thesis includes two sections—review and research report. In the review section, informations about high performance liquid chromatography-chemiluminescence detection (HPLC-CL) such as history, principles, HPLC-CL equipment and the main chemiluminescence reaction systems used in HPLC-CL was summarized. In the next research report section, four applied reports about HPLC-CL for determination of sinomenine hydrochloride(SIN), Ellagic acid(EA),Naringin and Potassium Sodium Dehydroandroan drographolide Succinate (DAS-K) were reported, respectively.Along with the development of CL technology, we get a full-scale understanding about the CL. As an important analytical method, CL has the merits of great sensitivity, wide dynamic range, simple equipment and easy automatization. However, the disadvantage of poor selectivity limited its application in many areas. So, the combination between HPLC and CL became a necessary consideration. Compounds were separated on the HPLC column and next mixed with CL reaction reagents, the lights emissed from the substances involved in this reaction course were recorded and conducted. Thus, the frame of HPLC-CL came into being. This analytical method has both excellences of CL and HPLC, at the same time, succeed in conquering the disadvantage of CL. Compare to some familiar detectors used in HPLC, such as UV detector, CL detector could achieve higher sensitivity for determination of many substances.Since the first report of HPLC-CL technique by Hartkopf, et al in 1974, this technique has developed rapidly. It has become a powerful tool for analysis of trace and ultra-trace ingredients and was widely used in the fields of metallurgy, chemical industry, pharmacy, environment, life science, biology and clinical medical science. The following four reports are our researching results.1. Determination of sinomenine hydrochloride by Reversed-Phase High Performance Liquid Chromatography with Chemiluminescence DetectionAn analytical method consisting of HPLC separation and chemiluminescence detection for determination of sinomenine hydrochloride(SIN) is described. It is based on the chemiluminescence reaction of KMnO₄-Na₂S₂O₃-SIN. The chemiluminescence...