Effects Of Different Flow Field On Cell Monocyte-endothelial Adhesion And The Changes Of VE-cadherin Expression And Distribution In Endothelial Cells After Cell Adhesion

Hemodynamics ranks extremely in the pathogenesis of atherosclerosis. Recently, the findings of researches demonstrate that the changes of shearing force result in onset and development of chronic vascular disease like atherosclerosis by influencing vascular endothelial cells (ECV) behavior, such as permeability of junctions between endothelial cells, proliferation and apoptosis of the cells, and especially, influencing changes of their stickiness to monocyte because it is crucial pathological process in early atherosclerosis. The animal model for atherosclerosis produced by our experiment group, proved that the changes in hemorheology after arterial stenosis, especially the appearance of local turbulent are close related with formation of atherosclerosis. To approach the relationship between them further, we produced the fluid model in vitro firstly, and simulated physiological lamina flow and pathogenic turbulent flow, and then observed the effects of different flow field on cell adhesion between monocytes and endothelial cells and the changes of VE-cadherin expression and distribution in endothelial cells after cell adhesion.