The Effect Of Latrunculin-B On Rabbit Uveoscleral Outflow

Object: To determine the effects of latrunculin (LAT-B )on intraocular pressure (IOP), aqueous humor flow (AHF), uveoscleral outflow and the permeability of sclera. This study was to prefer a new idea and theoretic basis about the effect of cytoskeletal reagents on the clinical therapy of glaucoma and the drug delivery for posterior segment eye diseaseMethod: Topical LAT-B was administered to one eye, and vehicle to the other. IOP was measured by Goldmann tonometry. the IOP were measured at 1 hour before administration and 1h, 2 h, 3 h, 4h, 5h, 6h, 24h after administration respectively; the permeability of sclera was determined by simulation IOP filtration system; the change about the expression of MMP2 and TIMP-2 were detected by the method of immunohistochemistry and immunofluorescence was detected by instillation of FITC-BSA into anterior chamber, at 2 h, 4 h,6h,8h, 10h ,2 rabbits were killed and frozen section of the eyes were done, and then the fluorescence intensity of ciliary body, epichoroidal space, anterior and posterior sclera were detected respectively. Results:Lat-B could decrease the IOP , compared with the IOP before drug administration, the difference was significant(P<0.05),and this effect occurred within 1 hour, some IOP effect may have remained at 24 hours, but the IOP of BSS group and DMSO group were not decreased. Compared with BSS group and DMSO group, the filtration of LAT-B group were increased (P<0.05). the expression of MMP2 and TIMP2 were increased after drug administration of LAT-B. the fluorescence intensity of ciliary body, epichoroidal space, anterior and posterior sclera after drug administration of LAT-B were higher than that of BSS and DMSO(P<0.05), there was no difference between BSS group and DMSO group(P>0. 05). Conclusion:...