The Effect Of Nitric Oxide Synthase/Nitric Oxide In Vascular Endothelium Damage Induced By Alcohol

Objective: In the present study, we examined the injuring effects of vascularendothelium induced by alcohol; we also examined injuring effects of alcohol on cultured human umbilical vein endothelial cell ( HUVEC-12 ) and explored whether the injuring effects of alcohol were related to both changes of ADMA level in serum or culture medium and lipid peroxidation.Methods: The experiment was divided into two parts. In the one part, Sprague-Dawley male rats weighing about 250g were actively given the three doses of alcohol (14V/V, 28V/V, 56V/V) by intragastrication in 1mL/100g, one time per day for two months. On the60th day, the blood samples were collected from femoral arteries, centrifuged(3500rpm,10min) and separated immediately. Serum concentrations of glucose (GLU), total cholesterol (TCHO), triglyceride (TG) (spectrophotometric method) were determined. In the case of the time-effect of alcohol, serum concentrations of GLU, TCHO, TG at 20^th, 40^th and 60^th day after alcohol being given were determined. Serum concentrations of MDA (thiobarbituric acid method), NO (Griess method) and ADMA (HPLC) levers were determined. Serum activity of NOS (biochemical approach) was determined.The experimental rats were randomly divided into 7 groups: (1) negative group, rats were given with equal volume of distilled water; (2) the alcohol groups, rats were given with different alcohol at concentrations (Hongxing Erguotou, 14V/V, 28V/V, 56V/V); (3) high fat diet group, rats were given with high fat diet; (4) high fat diet & alcohol at middle concentrations group (28V/V); (5) VitE (100mg/kg) protective group, rats were given with...