The Effect Of Flavonoids From Seed Of Hippophae Rhamnoides L. On Blood Lipid Metabolism In Climacteric Rats And Its Mechanism

Residues of seed from Hippophae rhamnoides L. are rubbishes of primary manufacture in the industry; they contain flavonoids, kinds of bioactive ingredients. The depth exploiture for resourse of seabuckthorn is to explore the function of the flavonoids by different levels from animal model in vivo to cell model in vitro, especially blood lipid metabolism and its mechanism in climacteric and climacteric obesity.Menopausal rats with aging were administered orally with FH at dose of 50,100,150mg/kg·d^-1,compared with water was given to the control group every day and with nilestriol at 0.4mg/kg was given to positive control group once a week for 12 weeks. The results showed that oral administration of 150 mg/kg·d^-1 FH to climacteric rats exhibited significant reduction in the LH level, the blood total cholesterol(TC) and triglyceride(TG): 25.2% and 30.7%. FH could enhance T-AOC and SOD in serum and liver significantly, and reduce MDA at the same time. But FH had little effect on food and water intake in climacteric rats, and also had little effect on weight and viscera weight quotiety.All the results mentioned above indicated that FH could decrease the level of LH, but had no effect on estrogen. It could also enhance the antioxidative activity and improve the climacteric rats' lipid metabolism, and FH could protect the aging ovary cell.In the above study, FH could decrease the level of LH, which indicated that it is necessary to pay attention to the effect on LH in health female rats. Thenceforth, we studied the effect of FH in health female rats. The rats were administered orally with FH at dose of 150 mg/kg·d^-1, while distilled water were given to the control group. The results showed that FH had no effect on the level of LH, and had little effect on the blood total cholesterol, triglyceride and the weight quotiety of ovary, uterus and liver in health female rats. FH controlled the weight of health female rats, but the effect mitigate in the long time.Women become fat easily in menopause because the hormone levels change during the time. In this test the bioactivity of FH on climacteric rats in obesity have been studied with diet-induced obese climacteric rats, which were made by feeding with high fat and sugar diet for 8 weeks, and high sugar diet for 12 weeks. The rats were administered orally with FH and Hippophae rhamnoides oil (HRO) at dose of 150 mg/kg·d^-1 and 2mL/kg·d^-1, comparec with water every day and nilestriol at 2 mg/kg·d^-1 were given to the control group and positive control group. The results indicated that FH and HRO did not upgrade the level of insulin and insulin sensitivity index (ISI), and also the blood glucose level. But FH and HRO caused reduction in the blood fructosamine: 17.57％and 23.88％. FH and HRO could decrease food and water intake to some extent in climacteric fat rats, especially FH. FH could decrease serum triglyceride level and free fatty acids (FFA), while HRO exhibited significant reduction only in FFA. Both could improve blood lipid metabolism in climacteric fat rats.All the results above and the previous researches showed that FH and HRO could reduce triglyceride,total cholesterol and FFA levels, while control the weight of climacteric fat rats through improved food and water intake. Both FH and HRO could improve blood lipid metabolism in climacteric fat rats.To study the pharmacological mechanism about lipid metabolism of FH, based on the experimental results in vivo, the effect of FH on hepatic lipase (HL) was studied by enzyme method. The results of the study for HL showed that FH had no effect on HL activity, which indicated that FH did not improve lipid metabolism by increasing the HL activity, and the mechanism need to be studied further.