The Extraction Of Huperzine A And Study On Pharmaceutical Effect And Pharmacology

Huperzia serrata (Thunb.) Trev. that experiment to choose was obtained originally from the some pharmaceutical factory of ChongQin . The Two factors test mensurate in sour kinds, sour pH value ,distill time, basification pH value,extraction times,extraction bulk ratio etc .In course of distilling Huperzine A from Huperzia serrata (Thunb.) Trev., L9(34) orthogonal experiment was advised to optimize four parameters of sour distilling dulk ratio,sour distilling times,sour distilling time and sour distilling temperature for obtaining the highest distilling ratio.Distilled Huperzine A was cranked out drug to feed mice for one month.then pharmacodynamics experiments were done in themselves.we adopted mouse skip platform experiment and mouse shunning dark experiment to examine pharmaceutical effect of Huperzine A.After experiment,mice were killed in different kinds ways.The way of instant cutting head was adopted in acetylcholine mensuration experiment;They were freezed in liquid nitrogen. They would be killed and taken the brain away in the freezing situation.then more than crude material nine time of volumes 2% perchloric acid was soaked .They were in the freezing preservation.at lastthey were crashed in cool water with the ultrasonic wave breaking mAchine in condition of 600 watt and five minutes.Breaking the neck way to execute under the normal temperature was used in acetylcholinesterase and choline acetyltransferase activity.Brain was taken out on the ice tray and soaked in more than crude material nine time of volumes physiological saline.Brains were crushed...