Investigation Of The Mechanisms Of Dermal Multipotent Stem Cells In The Processes Of Wound Healing

Stem cells are defined as clonogenic cells capable of both self-renewal and multilineage differentiationm, which means that these cells can be expanded in vivo or in vitro and differentiated into the desired cell types. Stem cells are generally divided into embryonic stem cells and adult stem cells, and both of them play very important roles in cell-replacement therapy, gene therapy, developmental biology, pharmacology and toxicology, et al. Adult stem cells are now isolated from many organs, and some recent reports suggest that functional plasticity of adult stem cells might be greater than expected.The use of adult stem cells will circumvent the ethical dilemma surrounding embryonic stem cells. Among all the stem cells, mesenchymal stem cells (MSCs) have been proved to have the potential to proliferate and differentiate into multilineage of cells. In our previous research on adult stem cells, dermal multipotent stem cells (dMSCs) were isolated successfully from rat skin. Both topical and systemic transplantation of dMSCs can accelerate the healing process in rats with a simple wound. These results suggest that neonatal dMSCs might participate in the regenerative process of the injured dermis and be an alternative choice for cell-based therapies of skin injuries. To further study the molecular mechanisms of dMSCs in wound healing, qu's group constructed the library to screen the differentially expressed genes in dMSCs stimulated by early-phase wound fluids using suppression subtractive hybridization (SSH) technique.In our experiments, dMSCs were routinely isolated, purified, expanded and identified. Then, wound fluids of 1st, 2nd,3rd and 4th day were collected, and the responses of dMSCs to wound fluids were investigated. After that, the insert fragments of 485 clones in the library were expanded by PCR method, and the PCR products were made into a genechip to identify the differentially expressed genes in the library. The highly expressed DNA clones were sequenced, and their significances were primarily studied. Finally, we investigate the proliferation promotion effects of SLPI on dMSCs and the possible mechanisms.