The Effect Of OxyHb On VSMC And VAF As Well As The Intervention Of Ecdysterone

Background: Chronic cerebral vasospasm(CVS)is the major cause of the morbidity and mortality following subarachnoid hemorrhage(SAH). The research of the mechanism of CVS has been lasted for several decades but it remaines unclear till now.It is indicated in experimental and clinical studies that pharmacological treatment aimed at persistent constriction of smooth muscle could meliorate CVS in some extent or in the early phase ,but could not take effects on severe CVS . It was observed in the arteries of patients and animals following SAH that there were necrosis, proliferation and thickening of muscle layer. Studies also indicated that Vascular advential fibroblasts was involved in the vascular remodel following SAH, although the mechanism is unclear. Previous studies focused on the active constricting of vascular smooth muscle cells (VSMC) and little attention have been paid to the characteristic of proliferation, migration and apoptosis of cells within the vascular. Animal model were commonly used in related studies, but the results were often quite different from each other and controversial still remains.Object : Oxyhemoglobin (OxyHb) was used as pathogeny on culturing VSMC and VAF from rat aorta to mimic SAH status in vivo .We try to explore the mechanism of CVS and effectiveness of ecdysterone (EDS) in CVS by observing pathomorphological changes , proliferation status, migration ability, apoptosis status, [Ca²⁺][ and proliferative effects of corresponding VAF conditioned medium on VSMC. Along with that we also pilot study the effect of T- type calcium channels in SAH.Methods:1. Pure VSMC and VAF from rat aorta were cultured in vitro, and the purity was confirmed by immunocytochemistry;...