Function Of CD147 In Murine Hepatocarcinoma Hca-F And Hca-P Cells Lymphatic Metastasis In Vivo

Invasion and metastasis are the most important biological character of malignant tumor and factor of malignant tumor causing death. The study of tumor metastasis mechanism is important significance for the early diagnosis and therapy of tumor metastasis. The modes of tumor metastasis have lymphatic metastasis, hematogenous metastasis and implantation metastasis. Above 50% tumors occur lymphatic metastasis, but the mechanism of metastasis has not yet been thoroughly explored. Several previous studies have demonstrated that tumor metastasis is not only related with organism microenvironment, but also associated with tumor cells property. The glycosylation of cell surface glycoprotein play significant roles in regulating tumor metastasis.Disassemble of extra cellular matrix are regarded as the early signal of invasion and metastasis.Matrix metalloproteinases can degrade many extra cellular matrix ingredient including Fn,Ln,collagen and fundus membrane glyprotein. MMPs are a kind of protein hydrolyzenzyme that can destroy adjacent tissue and play an important role in tumour metastasis and blood vessel formation . The related CD147 is a highly glycosylated immunoglobulin superfamily transmembrane protein ,which is enriched on the surface of many malignant tumor cells and can activate tumor cells to produce MMPs, so is related to invasion and metastasis of tumor.CD147 is a highly glycosylated immunoglobulin superfamily transmembrane protein, which is enriched on the surface of many malignant tumor cells and a membrane spanning molecule. As a result of heterogeneous N-glycosylation, CD147 exists in both a highly glycosylated form, HG-CD147 (~40-66kDa) and lowly glycosylated form, LG-CD147 (~33kDa). But the relationship of CD147 glycosylation and tumor metastasis is not clear. CD147-mediated MMP induction could be a common mechanism in physiological and pathological situation, such as tissue remodeling, development, reproduction, tumor, arthritis and ulceration. On the other hand, CD147 is also highly expressed on the cell surface of various tumors. It has been confirmed that the expression of CD147 is high in many malignant tumor. Later studies have shown that CD147 level was detected in numerous malignant tumors, such as lung cancer, hepatoma, neurogliocytoma, esophageal cancer, laryngeal...