The Expression Of PPARγ In The Liver Of Rats With Nonalcoholic Fatty Liver And The Effect Of Ganzheng Fang

Objective: The prevalence of nonalcoholic fatty liver disease(NAFLD) is increasing, unfortunately,up to date little is known about its pathogenesis and there are few effective drugs to heal it. To observe the changes of rats with NAFLD in aspects of blood lipid, lipid peroxidation system, hepatic function, expressions of peroxisome proliferator-activated receptor gamma (PPARγ) protein and mRNA, pathological change of liver tissues et al , and to know the curative effects and mechanism of Ganzheng Fang in treating rats with NAFLD.Methods: Thirty male Sprague-Dawley rats were divided into three groups randomly with 10 in each group: Control group were fed with normal diet; Model group and treatment group were fed with fat-rich diet to establish NAFLD models. From the 13th week of model establishment, the rats in normal group and model group were treated with distilled water of 10 ml/kg, once a day; while 10 ml/kg GZHF solution given in treatment group, once a day.Sixteen weeks later, all the rats were sacrificed to detect levels of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC) and triacylglycerol(TG) by using automatic biochemistry analyzer. Serum tumor necrosis factor alpha (TNFα) was tested with radioimmunoassay. Kit was used to detect TC, TG, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in liver homogenate. The level of steatosis was evaluated under light microscope after haematoxylin-eosin (HE) staining and inflammation activity was also scored.The immunohistochemical staining,Western blotting and reverse transcription-polymerase chain reaction (RT-PCR)...