Font Size: a A A

Immune Response In Cerebral Cortex Of Ischemic Mice And Changes Of T Lymphocyte Subsets In The Spleen

Posted on:2007-09-12Degree:MasterType:Thesis
Country:ChinaCandidate:H ZhaoFull Text:PDF
GTID:2144360185470745Subject:Physiology
Abstract/Summary:PDF Full Text Request
Background and PurposeMany key evidences demonstrate that immune response correlate with a lot of central nervous system (CNS) disorders such as stroke, brain trauma, tumor, ischemia and neurodegenerative diseases. Acute cerebral arterial thrombosis is one of the nervous system diseases. There are many evidences have shown that the immune response contributes to ischemic brain injury. Activation of endogenous microglia play a role in the pathogenesis of ischemic brain damage, leukocytes from peripheral blood are also contributing to the CNS ischemia by leaving the periphery and quickly penetrating within the CNS. Meanwhile, changes of cellular immune response in the periphery have been found. If the quantity of T lymphocyte subsets are abnormal, dysregulated immune response will induce many diseases. There are many studies on the relationship of T subsets and ischemia. To observe the function of lymphocyte subsets during ischemia, We studied the penetrating of lymphocyte in the brain and changes of spleenic T subsets in mice photothrombosis model.Materials and MethodsWe selected healthy male adult Balb/c mice as research objects. Seventy two mice were divided into groups randomly: control group(n=24), Rose bengal group(n=24) and ischemia group(n=24), every group was divided into four subgroups according to the different sacrificed time point: that is 6h, 1d, 3d and 7d. The focal cerebral ischemia was induced: injection of the photosensitive dye Rose bengal via intraperitoneal, subsequent focal illumination of the brain with a cold light source through the intact skull led to focal cortical infarct. Half an hour before the sacrificed time point , The spleen was aseptically removed, and...
Keywords/Search Tags:cerebral ischemia, immune response, flow cytometry, T lymphocyte subsets
PDF Full Text Request
Related items