Study On Application Of High Performance Liquid Chromatography With Restricted-Access Media For Biopharmaceutical Analysis

A process of biopharmaceutical analysis with high performance liquid chromatography (HPLC) generally involves four main steps: sample preparation, analytical separation, detection and data handling. In the biopharmaceutical analysis field, sample preparation is often considered as the time-limiting step. Indeed, the extraction techniques commonly used for biopharmaceutical analysis such as liquid-liquid extraction (LLE) and solid-phase extraction (SPE) are achieved in the off-line mode. With these techniques, we can not establish a more accurate, more precise, simpler and quicker method for biopharmaceutical analysis and we can not carry out the determination of unstable drugs either.In order to perform a high throughput analysis, efforts have been engaged in developing a faster on-line sample purification process. Among different strategies, the introduction of special extraction sorbents, such as the restricted access media (RAM), allowing the direct and repetitive injection of complex biological matrices samples, represents a very attractive approach. Integrated in a HPLC system, these extraction supports lead to the...