The Primary Experiment Research On The Mature Epidermal Cell Dedifferentiation To Stem Cell In Different Induction Factors And Isolation Culture And Identification Of Adult Epidermal Stem Cell

Objectives:1.To grasp isolation culture and identification of adult epidermal stem cell and mature epidermal cell in vivo and investigate their biology properties.2.To study differential effects of multiple induction factors to adult epidermal cell.3.To practice the possibility about mature epidermal cell differentiation to stem cell by epidermal graft model during wound repair process.Methods:1.Epidermis was obtained by digestion adult human foreskin with protease and dissociated into single cells with trypsin and ethylene diamine tetraacetate (EDTA). These single epidermal cells were inoculated onto human collagen IV coated flasks at 37 ℃ in a humidified atmosphere containing 5% CO2, then adult epidermal stem cells and mature epidermal cells were gotton by distinction-adherent method and they were identified with different levels expression of keratin by using immunocytochemical...