Study On Therapy For Asthma By Aerosolizing Delivery Of CpGODN Mediated By A New Type Cationic Liposome

Objective(1) Part I To prepare polyamine cationic liposome (PCL) ,and trans-fect it into mammlian cell to select a novel PCL with high transfection efficiency and low cytotoxicity and optimize the condition of transfection.(2) Part II To transfect CpGODN mediated by the PCL selected by aerosolizing delivery to asthma Balb/C mice to study on therapy for asthma.Methods(1) Part I Preparation and selection polyamine cationic liposome with high transfection and low cytotoxicity: PCL with polyamine cationic lipids TC-Chol, DC-Choi and neutral phospholipid DOPE at a certain molar ratio was prepared, the plasmid PIRES2-EGFP which mediated by PCL was transfected into mammalian cells,and the expression of EGFP...