The Expression Character Of Nucleostemin Gene In Liver Cancer Tissues And Cell Lines

Nucleostemin(nucleus stem cell factor, NS) is a cell factor which was discovered in rat embryo stem cells, the rat central nervous system (CNS) stem cells and rat primitive bone marrow cells by McKay and Tsai in 2002. The NS protein can interact with p53 and exists in the stem cell nucleus. The research indicated that All detected malignant tumor tissues,including human gastric cancer, bladder carcinoma, pancreatic cancer, osteosarcoma cells, benign tumor tissues, and benign hyperplastic tissues had high levels of nucleostemin expression. Nucleostemin was also expressed in human placenta tissue at a high level. The discoveries of NS in two kinds of cells suggested this gene play the similar roles in these cells.The mortality rate of Hepatocarcinoma (Liver cancer) is the fifth-highest in the world. The expression of NS gene is rarely reported in liver cancer. Therefore, liver cancer is a great model for the NS research. In this article, the NS's expression was studied in liver cancer organizations and the cell lines to discuss the physiological significance of the NS gene in liver cancer.First, we got the clones of the fragment and the whole CDS of NS gene in the cDNA from liver cancer. Then we determined the expressions of NS in 7 kinds human embryo organizations by PCR. Again, the expressions in 40-pairs of liver cancers, normal tissues beside these cancer organizations and three kinds hepatocarcinoma cell lines (HepG2: human liver cancer cell line with low- transfering ability; SMMC-7721: human liver cancer cell line, the transfering ability compares HepG2 to be high; MHCC: human liver cancer cell line with high-transfering ability)were studied by the same method. The results indicated that the expression is higher in liver cancer cells with stronger proliferation than those cells with lower proliferation, in transcription level. In the translation level, we extracted the cell protein from the above three kinds...