Production Of A Monoclonal Antibody Against Human Pancreatic Cancer Cell And Primary Study Of Radioimmunoimaging

Objective: To production and identify monoclonal antibody (McAb) of human pancreatic cancer associated antigen,and to study radioimmunoimaging of 99Tcm labeled McAb2F4A7 in nude mice bearing human pancreatic carcinoma.Methods: Balb/c ices were immunized with human pancreatic cancer cell line Patu8988 and the lymphocytes hybridoma technique was used for McAb preparation. The McAbs were screened by indirect ELISA;The tissue specificity of McAb were identified by flow cytometry and immunohistochemical ABC staining;McAb was typed by reaction with subclass-specific sheep anti-mouse antibody; Being labelled With 99Tcm,McAb 2F4A7 was used to the radioimmunolocalization triais.9 nude Mice bearing human pancreatic carcinoma xenograft were used in the trial when xenograft were as big as almost 1 cm3 after 8 Weeks of growing. 99Tcm -McAb 2F4A7 was injected into peritoneal cavity.2h, 4h, 6h later,the nude mice were examined with SPECT to measure the radioactivity of tumor xenograft and the normal organs.Results: one hybridoma cell line (2F4A7),which seecretes monoclonal antibody against pancreatic carcinoma,was established .Using lsoStript kit,it was revealed that McAb2F4A7 belonged to IgGl subclass and K subtype, its titer in ascites was 1:1.6×104 Flow cytometry showed that Mab 2F4A7 was postive with pancreatic cancer cells , while did not react with liver cancer,cervical cancer,lung cancer,gastric cancr,breast cancer cell.;MTT assay proved that the cytotoxicitis of effective groups were higher than control groups; Immunohistochemistry results showed that McAb 2F4A7 reacted with...