Study Of Expansion And Immunosupressive Property Of Mesenchymal Stem Cells (MSCs) From Patients With Systemic Lupus Erythematosus In Vitro

Objective: To investigate expansion of mesenchymal stem cells (MSCs) from patients with systemic lupus erythematosus in vitro and their immunosuppressive property in mixed lymphocyte reactions . Methods: The MSCs were isolate from the bone marrows of 5 SLE patients and 1 healthy volunteer and were adhesively cultured in vitro. Lymphocytes were prepared from the peripheral blood of 24 patients with SLE and 1 healthy volunteer. The lymphocytes from the healthy volunteer (stimulating cells) were used to stimulate the lymphocytes from the SLE patients (reacting cells) and the two lymphocytes were cultured together as bidirectional mixed lymphocyte reactions (MLRs). MSCs were cultured with MLRs while the control group included MLRs alone. Carboxyflourescein diacetatesuccinimidyl ester (CFSE) was used to mark the reacting cells in MLRs. After cocultured 60h, FCAS was used to investigate the change of reacting T cells.Result: MSCs were successfully separated from 5 SLE patients. However, MSCs from 3 patients were able to grow well. The MSCs of...