Effect Of RhPRL-NK-92 Cells Against Human Cervical Carcinoma Cells In Vitro And In Vivo

ObjectiveTo evaluate the anti-tumor activity of NK-92 and rhPRL-NK-92 cells against human cervical cancer cells both in vitro and in vivo in NOD/SCID mice inoculated with human cervical carcinoma.Methods1. In vitro experimentsTo assess the effect of irradiation on NK-92 and rhPRL-NK-92 cells, cells were exposed to different doses of radiation and assayed for proliferation and by cell count using trypan blue exclusion. The cytotoxic activity of NK-92 and rhPRL-NK-92 cells (irradiated and nonirradiated) against targets was measured by a standard 4h ⁵¹Cr-release assay or MTT. Flow cytometric measurement was used to examine CD56 expression on NK-92 cell surface.2. In vivo experimentsTo determine in vivo cytotoxicity of irradiated NK-92 and rhPRL-NK-92 cells, NOD/SCID mice were challenged with human cervical cancer cells and treated with irradiated NK-92 and rhPRL-NK-92 cells. Mice were monitored regularly for tumor growth and length of survival. To control for any possible toxicity of the NK cells, irradiated NK-92 and rhPRL-NK-92 cells were injected into NOD/SCID mice, and the animals were monitored for survival and any tumor growth. Results1. 400y dose of irradiation could effectively control cell proliferation of NK-92 cells...