The Method For Determination Of DNA In Capillary Electrophoresis And Its Application

Capillary electrophoresis (CE) is a relatively new separation technique compared to the well-known chromatographic procedures, such as HPLC and gas chromatography separation of a complex array of large and small molecules, including maromolecules proteins. Sample introduction is accomplished by immersing the end of the capillary into a sample vial and applying pressure, vacuum or voltage. High electric field strengths are used to separate molecules based on differences in charge, size and hydrophobicity. Depending on the types of capillary and electrolytes used, the technology of CE can be segmented into several separation techniques, the forms used often are capillary zone electrophoresis(CZE) and capillary gel electrophoresis(CGE).CZE include cross-link capillary gel electrophoresis and no gel sieving capillary electrophoresis. Fundamental to CZE are homogeneity of the buffer solution and constant field strength throughout the length of the capillary. Fundamental to CGE are homogeneity of cross-link gel or linear high weight mass solution. The analytes are prepared by the differences in their sizes.Polyvinylpyrrolidone(PVP) is the main object of this experiment. We reported linear high weight mass solution as a sieving medium in capillary was used to set up mode of no gel sieving capillary electrophoresis and dynamic coating zone capillary electrophoresis to separate and quantitate DNA, then Y chromosome azoospermia factor microdeletions was performed for patiens with primary azoospermia and with oligozoospermia and the circulating cell-free RNA quantity of cancers were evaluated.Part I Establishment a method for Separating DNA fragments by no-gel sieving capillary electrophoresis in using polyvinylpyrrolidone as sieving mediaPolyvinylpyrrolidone (PVP) is used to separate pUC19DNA/Mspl(HpaII) fragments through no-gel sieving capillary electrophoresis. The optimal separation condition was choosed by adjusting PVP concentration, pH value of running buffer, voltage and capillary temperature. Under optimal conditions, pUC19DNA/Mspl(HpaII)...