An Experiment Study On Repair Of Peripheral Nerve Defects By Transplantation Of Neural Stem Cells

Objective To investigate the possibility of repair of peripheral nerve defects by transplantation of NSCs, so as to provide reference evidence for the establishment of a new method of promotion peripheral nerve regeneration.Methods â‘  To establish a culture system of NSCs: Cells were collected and cultured from fetal(embryonic aged 12 days) and newborn(aged 1 days) SD rat cerebral cortex. Their self-renewal ability, expression of Nestin and multipotent of differentiation were identified with BrdU, Nestin, MAP2 and GFAP immunocytochemical staining. â‘¡ To establish a culture system of SCs:...