Study On Mechanisms Of Anti-hepatofibrotic Effects Of Compound Astragalus Extract

Hepatic fibrosis is a kind of common disease that is associated with a significant morbidity and mortality, which is a major worldwide health care burden. For it is the common pathological feature in the development of chronic liver diseases, the key to control these diseases would be preventing, treating and reversing hepatic fibrosis. Compound Astragalus Extract (CAE) is the combination of active compound extract from the Astragalus. Previous study from our laboratory showed that CAE has significant activating blood to resolve stagnation, anti-inflammatory, anti-oxidative, anti-hepatic injure, anti-hepatofibrotic and immuno-modulated effects. Based on the previous study in our laboratory, this article was designed to explore the mechanisms of anti-hepatic fibrosis of CAE in vivo and in vitro. The main contents are divided into the following sections:1. Inhibitory effect of CAE on HSC functionIn vitro models for proliferation and collagen production of a cell strain named HSC-T6 stimulated with serum and cytokines (PDGF-BB,TGF-β₁) respectively were established, which were measured by MTT and ³H-proline incorporation. The result of concentration -inhibition of CAE on proliferation and collagen production in vitro showed as the followings: (1) CAE (10,20,40, 80 å’Œ 160 mg·L^-1) inhibited the proliferation of HSC-T6 cells ,stimulated by 10% NBS, concentration dependently, so did CAE on collagen production (2) CAE (5,10,20,40 å’Œ 80 mg·L^-1) concentration -dependently inhibited PDGF-BB (10 μg·L^-1 ) -driven proliferation of HSC-T6...