Expression Of CK5,CK34βE12,S100P,CK8,SMA And E-cad In Intraductal Proliferative Lesion And Valuation Of Diagnosis

Purpose To explore the morphologic and immunophenotypic characteristics of intraductal proliferative lesion in resected specimen of breast carcinoma and the correlation with invasive carcinoma. Methods 36 cases of breast carcinoma with much tissues beside the carcinoma in resected specimen and 26 cases of benign lesions in breast were randomly selected at the same period. Expression was detected by immunohistochemical staining using antibodies to CK5, CK34βE12, S100 protein, SMA, CK8, E-cad. Results Among the 36 cases in the group of breast carcinoma, 28 cases had intraductal proliferative lesions, separate or multiple, including 7 cases of UDH, 12 cases of CCC, 4 cases of ADH, 24 cases of DCIS, 10 cases with the lesions more than two, 2 cases with all four lesions; 23 cases of UDH and 9 CCCs were contained in the benign lesion group. Immunohistochemical staining showed that CK5 was much expressed in glandular epithelial cells of florid UDH, and the expression decreased obviously to lost completely in ADH, DCIS and IDC. CK34βE12 expression was alike to CK5, but strongly than CK5, and both of them did not overlap completely. CK5 and CK34βE12 have some value of differential diagnosis for different IDPL, and CK5 is more sensitive than CK34βE12. S100 protein expressed in myoepithelial cells and proliferating glandular epithelial cells, which was alike to CK34βE12, but was negative for SMA. The myoepithelial cells were lost in reactivity to S100 protein in 11 of 24 cases of DCIS of grade III, but kept strong positive for SMA. E-cad reappeared in...