| Sulfur mustard (HD), a potent vesicant agent, is nortorious by its cutaneous, ocular and respiratory toxicity. HD possesses strong penetrability, permanence, hard precaution, complex disposal and easy recurrence. Presently, no sucessful measures are available in treatment of patients suffered with HD, except supportive therapy such as anti-inflammatory.In consideration that skin is the major target for HD, to improve the prophylactic and therapeutic level for HD, the research on mechanism of skin injuried by HD and antidotes became a hotspot. With the application of new technology, greater development has been made by foreign researchers in molecular mechanism of skin vesication, cellular apoptosis, cytotoxic mechanism and new ways of promoting skin concrescence. In recent years, researchers have investigated the effects of HD on inflammatory medium, cytokines and chemokines in keratinocytes cultured in vitro respectively, which, however, can not fully revealed the molecular mechanism of HD due to its characteristics of multi-levels and multi-targets. The changes in the protenomic of mouse skin treated with HD have not been well understood. Our research aims at the identification of different proteins and possible mechanism of HD-induced skin injuries, in the hope of providing a theoretical basis for HD prevention and cure.The results are as follows:1. Identification of differential proteins of pinnal skin of mice poisoned by sulfur mustardTo investigate the molecular mechanism of skin lesion in sulfur mustard-poisoned mice by differential proteomic analysis. Mouse ear were isolated from normal animals and animals pre-treated topically with sulfur mustard (5 μL, 160 g·L-1). The total protein samples used in 2-D electrophoresis were prepared by discarding soft bone and ultracentrifugation. The protein maps were compared with ImageMaster 5.01 analysis software and analyzed with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). The...
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