The Study On Bone Marrow Stromal Cells Transfected With Noggin Gene Differentiating Into Neuron-like Cells In Vitro

ObjectiveStroke is a devastating desease with loss of neurons and breakdown of reflex arc.It has a high incidence and mortality.The most effective and ideal treatment may be to rebuild the reflex arc, which need the new neurons.The discovery of the neural stem cells in mammals sheds new light on the best recovery from this terrible disease. However, neural stem cells are rare and unaccessible, which has become a most critical problem.Bone marrow stromal cell (BMSC) is a kind of the mesodermal stem cells existing in bone marrow.It is considered as an ideal sources of seed cells with the wide potential of differentiation and the advantage of being isolated and proliferated in vitro easily, which is a prerequisite for the autotransplantation. Up to now, there are many reports on cell's differentiation into neurons by inducer such as retonoic acid, β -mercaptoethanol and baicalin. No study has explore the differention of BMSC induced by noggin, an important neural inducer in embryos.Noggin was originally isolated from Xenopus embryos by Harland R.M. and Smith W.C. in 1992. It plays an important role in normal development,especially of inducing dorsal development. As an endogenous neural inducing signal, noggin not only can be found in early vertebrate embryogenesis but also in the central neural system (CNS) of adult mammalian .What's more, Noggin is also involved in other tissue's development processes, such as joint formation.Therefore, we design to construct an expression vector of Noggin and explore the differetiation of BMSC transfected with this vector. The result will promote the further research in the biological function of noggin gene, the stem cell therapy and gene therapy in cerebral disease.Methods1. After isolated by density gradient contrifugation with Percoll, the rats' bone marrow stromal stem cells were cultured with DMEM containing 20% fetal bovine serum in the plastic flastic. When the cells covered the flask, they were passaged with pancreatin. Their morphology were observed by microscope.