Study On The Protective Effects Of Heparin, Hyaluronic Acid And Trehalose In Cornea Preservation Medium

Cornea preservation helps the successful performance of penetrating keratoplasty. The methods of cornea preservation include cryopreservation, organ culture, moist chamber storage at 4℃ and intermediate term storage (storage at 4℃ in medium). Among them the intermediate term storage and organ culture are commonly used, but organ culture is only used in Europe. The intermediate term storage is a very simple way to follow. Now the well-known medium Optisol-GS which contains the basic medium, high polymers, antibiotics, buffer system and some other additives for better maintaining can protect the cornea during storage as long as 3 weeks, but 2 weeks is recommended. High polymers are important for maintaining the activities of the cornea under 4℃. Now chondroitin sulfate and low molecular weight dextran are the most common and effective combination. Considering the properties and biological activities of heparin, hyaluronic acid and trehalose, we apply them in cornea storage medium and explore their protective activities for cornea and the mechanisms. The points of my research include screening of the formula of the corneal preservation medium containing heparin, hyaluronic acid and trehalose, the protective effects of heparin and hyaluronic acid in cornea preservation medium and the preliminary study on the mechanisms of the protective effects.1 Formula screeningHeparin, hyaluronic acid and trehalose were added in basic preservation medium...