Protective Effects Of The Effect Parts Of Zingiber Officinal On ECV-304 Cells Oxidative Stress Injury Induced By Hydrogen Peroxide In Vitro

Objective: Vascular endothelium has attracted much attention of scientists and has been examined for its critical role in the preservation of normal vessel wall structure and function as endothelial cells control vascular permeability, vessel tone, coagulation, fibrinolysis and inflammatory responses accomplished by production of a variety of biologically active substances. Vascular endothelial cells injury, including structural injury and functional injury, is a postulated initial step in the pathogenesis of atherosclerosis. Expression of adhesion molecules and chemoattractant protein by actived endothelium at sites of vascular injury leads to the recruitment of monocytes adhere to the endothelium. In this process, hydrogen peroxide (H2O2), an active oxygen species plays a major role. The effect parts of zingiber officinal (EPZ) are extraction which extracted from zingiber contains gingerol and aetherolea. In animal studies, it was approved that EPZ has the effects of blood lipid regulation, anti-oxidation and decrease inflammatory factor's expression. This study is aimed to observe the influence of EPZ on ECV-304 cells oxidative stress injury induced by H₂O₂ and to investigate the potential mechanisms.Methods: 50 Wistar rats were randomly devided into 5 groups: normal group, EPZ(200mg/kg, 400mg/kg, 800mg/kg)group and lovastatin(40mg/kg) group. Take the blood serum which contains EPZ as experimental drug. The injury to ECV-304 was induced by exposure to H₂O₂(1000umol/L) for 4h, and then changed nutrient medium contains EPZ instead of H₂O₂ for 20h. Cell viability was determined by MTT. Monocytes were isolated from peripheral blood and the adhersion of monocytes to...