The Effect Of Carbon Dioxide Pneumoperitoneum On Intestinal Endotoxin/Bacterial Translocation In The Rat Of Peritonitis

【Objective】To examine whether carbon dioxide pneumoperitoneum increase intestinal Endotoxin/bacterial translocation in the rat of peritonitis and to learn the effect of different degrees of carbon dioxide pneumoperitoneum on intestinal endotoxin/bacterial translocation .【Methods】In this study , JM109 labeled plasmid PUC18 was given by gavage to detedct intestinal bacterial translocation. Rats were colonized with a strain of E.coli JM 109 labeled ampicillin-resistant plasmid PUC18, and then made into the model of acute chemical peritonitis by peritoneal inoculation with hydrochloric acid. 48 rats of peritonitis were randomly allocated to control groups with no pneumoperitoneum (n=16), low insufflation at 5 mmHg with carbon dioxide (n=16), and high insufflatin at 15 mmHg with carbon dioxide (n=16). The endotoxin content in femoral vein was detected every hour. Ampicillin–resistant strains with JM109 bearing plasmid PUC18, which was colonized to the animals previously, were identified by restriction digest and agrose gel-electrophoresis. All rats were killed at the end of the 3 hour experiment, blood samples from portal and mesenteric lymph...