Study On Antitumor Activity Of Naja Atra Venom Serum Of Rat

This paper studied the antitumor activities of Naja atra venom (NAV)Serum of rat and mouse in vivo & vitro.The researches on the antitumor activities of Naja atra venom Serum of Rat in vitro revealed that the growth of K₅₆₂,H₂₂,B₁₆,SGC-7901,ECV₃₀₄ cells were inhibited by Naja atra venom Serum of Rat in dose-and time-dependent manner. NAV inhibited the growth of mice implanted solid tumor U14 and S180 in a does-depend manner. The percent of inhibition of tumors exposed to the NAV for nine days was 94.31%,78.29%,27.05%,88.48%,80.12%,70.48%.The cytotoxic effects of NAVSR on K₅₆₂,H₂₂,B₁₆ tumor cells lines cultured in vitro were determined with trypan blue dye exclusion test method and growth curve method. The cytotoxic effects of NAVSR on SGC-7901 and ECV304 tumor cells lines cultured in vitro were determined with SRB test method and growth curve method . Immuno diffusion of agarose to Validate NAV was absorable by per os.NAVS induced significant growth arrest in various tumor cell lines cultured in-vitro and effectively inhibited in-vivo growth of mice implanted solid tumor of U₁₄ and S₁₈₀ for nine days.