Promoter Methylation Of Tumor Related Genes In Human Gastric Carcinoma

To discuss the methylation status in gastric carcinoma and discover the relationship between methylation of four genes(E-cadherin , hMLH1, APC and MGMT) and clinical-pathological characteristics of gastric carcinoma. We examined the promoter methylation of several tumor related genes in archived paraffin-embeded gastric carcinoma tissue and normal gastric epithelium.The results show that the rate of genes methylatlion is 43.4%(46/106)cases at one or more gene tested in 106 cases normal gastric epithelium, the rates of gene methylation are 72.6%(77/106)cases, 44.3%(47/106)cases, 20.8%(22/106)cases and 9.4%(10/106)cases at one or more genes, two or more genes, three or more genes and four genes tested in 106 cases gastric carcinoma, respectively. There are statistically significant difference in the rates of gene methylation between normal gastric epithelium and gastric carcinoma(P<0.05).The result indicate the promoter methylation of E-cadherin, hMLH1 and APC occurred more frequently in carcinomas than in normal epithelium. It was common event in carcinogensis and may be an important cause of gastric carcinoma.The methylation of related genes occurred more frequently in diffuse type of GC than in intestinal type of GC(P<0.05). The pTNM staging, differentiation and infiltration depth of GC is significantly associated with methylation of genes. There are no significant association with age, gender, Ming's classification and lymth vessel metastasis. It illustrate that promoter methylation of related genes in GC is a key factor in occurrence and development of GC.When it comes to specific genes, the methylation of E-cadherin is associated...