Effect Of Gene Transfer Of Human Interleukin-10 On Ischemia Reperfusion Injury In Immature Myocardium

Objective:To investigate the effect of adenovirus-mediated gene transfer of human interleukin-10 in immature myocardium in vivo after regional myocardial ischemia-reperfusion, and discuss the mechanisms.Methods:Twisty-four immature rabbits (three to four weeks old and weighting 420±30g) were randomly divided into three groups (8 animals/group): group A: control group; group B: receiving empty adenovec; group C: receiving adenovec-hIL-10 complex. The animals were anesthetized with intraperitoneal injection of pentobarbital (30mg/kg).Chest was opened and heart exposed. Left coronary artery was temporarily occluded for 30 min followed by 120 min reperfusion. In group A, heart was exposed but left coronary artery was not occluded. In group B and C, left coronary artery was occluded for 30 min followed by 120 min reperfusion. Parameters on cardiac function were measured before ischemia and after reperfusion. Animals were sacrificed at the end of 120 min reperfusion. Myocardium samples were harvested on 120 min after reperfusion, histopathologic observation was carried out and result were compared and analyzed; Gene product expression, TNF-αand IL-6 in tissue was quantified by enzyme-linked immunosorbent assay (ELISE); the expression of hIL-10,NF-κB and TNF-αin Myocardium was detected by using immunohistochemistry staining method. Apoptosis cell deaths in Myocardium were determined by in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick labeling (TUNEL) and apoptosis index (AI) were analyzed. Myocardiums MDA, SOD, MPO were also measured.Result:on 150min after gene transfer in group C, transgene expression of hIL-10 was detected by means of both ELISA and immunohistochemistry. Expression scores...